Evaluation of Phytochemical and Antibacterial Potential of Vernonia amygdalina and Elaeis guineensis on Some Bacteria Associated with Diarrhea

Background: Bitter leaf and palm oil combination in treating diarrhoea is common Africa. The aim of this study to determine the synergistic antimicrobial potential Vernonia amygdalina Elaeis guineensis on bacteria isolates from diarrheic stool.
 Methodology: One hundred fifty (150) stool samples Diagnostic Laboratory Rivers State University Teaching Hospital (RSUTH), Port Harcourt, were inoculated Selenite F broth, MacConkey, Nutrient Salmonella-Shigella agar plates using standard methods. Fresh dry bitter extracts prepared by mashing, weighing dissolving 100 grams(g) 150 g concentrations each milliliters(mls) ethanol sterile distilled water respectively. Undiluted mixture mixing 2 mls a beaker. Zero-point one milliliter (0.1ml) undiluted extract, their mixture, separately dispensed into appropriately labeled wells pipette for sensitivity test isolated bacteria. pH, temperatures phytochemical contents determined pH meter, thermometer spectrophotometric method.
 Results: Escherichia coli, Staphylococcus aureus Salmonella sp. isolated. temperature extract 7.2 26⁰C 6.7 respectively while was 6.9 26⁰C. extracts, combination, used as antibiotics did not show any sensitivity. phytochemicals identified saponins, alkaloids tannins carotene detected oil.
 Conclusion: It can be concluded that inhibit growth result lack bioactive components leaf. nature diluents extraction may have been right it would caused low concentration, dilution disfiguring molecules compounds prevented proper reaction between used.